HBL-100-CAS9 cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

HBL-100-CAS9 (Misidentified source) Order

Catalog#

YC-C013-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

HBL-100-CAS9

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HBL-100 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HBL-100-CAS9 cell line that can maintain long-term, stable and high expression.

Cutting Efficiency Validation

HBL-100-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the HBL-100-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HBL-100			STR Info (Cell bank) Cell Line:HBL-100
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	12	12		12	12
D7S820	8	12		8	12
D16S539	9	12		9	12
VWA	16	16		16	16
TH01	6	8		6	8
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	10	10		10	10

* Misidentified source

Yoshino et al (2006) notes that the cell line carries a Y chromosome and is therefore unlikely to come from the original donor. HBL-100 was originally reported to come from "Human milk ... obtained by hand expression from a nursing mother 3 days after delivery" (Gaffney, 1982; PMID: 6891286). See also "Misidentified Cell Lines" page on the ATCC website.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

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